Preparation of high specific antibody against human C1q/TNF-related protein-1 / 国际药学研究杂志
Journal of International Pharmaceutical Research
;
(6): 216-219, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-845783
ABSTRACT
Objective:
To prepare specific antibody against human C1q/TNF-related protein-1 (hCTRP1).Methods:
A C-terminal antigenic peptide of hCTRP1 was synthesized and injected into the Newzealand rabbits. The antibody was purified by affinity chromatography column.Results:
High titer antisera against the hCTRP1 was prepared and the antiserum's titration was over 1 64 000 by indirect ELISA. The specificity of the purified antibody by affinity chromatography was identified by Western blot and ELISA.Conclusion:
The high titer antiserum is prepared by injection for rabbits and the specificity of the antibody purified from the antiserum is determined.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of International Pharmaceutical Research
Year:
2014
Type:
Article
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