Interactions between chitosan nanoparitcles and cells by surface plasmon resonance technology / 国际药学研究杂志

ABSTRACT

Objective: Using surface plasmon resonance(SPR) technology to monitor the interactions between chitosan nanoparticles(CS-NP) and biomembrane. Methods: SPR signals induced by CS-NP, chitosan molecules and thymopentin (TP5) molecules in living C6 cells were detected and analyzed by the SPR-based cytosensor. Results: The signal began to increase within 30 min after the addition of CS-NP or chitosan molecules. These responses were strongly dependent on the concentrations of CS. However, the signal did not change after the addition of TP5 molecules. Conclusion: SPR technology can clarify the interaction between nanoparticles and biomembrane in real time.