Preventive effect of senecio cannabifolius less.II on perfluoroisobutylene inhalation-induced acute lung injury in rats / 国际药学研究杂志

ABSTRACT

Objective To preliminarily investigate the effect and possible mechanisms of Senecio cannabifolius Less. II (FHC- n) on perfluoroisobutylene (PFIB) inhalation-induced acute lung injury. Methods Totally 156 rats were randomly assigned to three groups the control group， the PFIB group and the FHC- H prevention group， with 32， 62 and 62 rats in each group respectively. The FHC- II prevention group were given FHC- II three times per day at the dosage of 340 mg/kg before PFIB exposure. 1 h after the last time of FHC- h administration，the FHC- i prevention group were exposured to gaseous PFIB (0.2 mg/L) for 10 minutes in a static whole-body exposure inhalation system. The survival rate of the rats were recorded at 1，2，4，8，16，24，48 and 72 h post PFIB exposure; the lung index and total protein content in bronchoalveolar lavage fluid (BALF) were measured at 1 h，2 h，4 h，8 h，16 h and 24 h; IL-1β and IL-8 in sera were assayed by enzyme-linked immunosorbent assay (ELISA) at 1，2，4，8 and 16 h post PFIB exposure and the histopathological examination of the lung tissue was performed at 8 h post PFIB exposure. Results FHC-II significantly reduced the content of the total protein in BALF，lung index and the levels of IL-1β and IL-8 in aera as well， and dramatically alleviated the histopathological changes in the lung tissue. Conclusion FHC- D demonstrates some preventive effect on PFIB inhalation-induced acute lung injury in rats.