Adjuvant effect of Epimedium polysaccharide for H1N1 influenza viruses split vaccine / 国际药学研究杂志

ABSTRACT

Objective To study the adjuvant effect of Epimedium polysaccharide co-immunized with H1N1 influenza virus split vaccine. Methods BALB/c mice were intramuscularly injected H1N1 virus lysate premixed with Epimedium polysaccharide G1, G4 and saline, respectively. The titer of serum antibody of mice was determined by ELISA two weeks after the initial immunization. The proliferation of T lymphocytes was measured by MTT assay and the levels of lymphocyte cytokines IFN-γ and IL-4 were determined by ELISA. CD4 +, CD8 +, CD3 + and CD19+ lymphocyte populations were determined by flow cytometry. Results Epimedium polysaccharides, G1 and G4, could rapidly induce the production of high levels of serum antibody against H1N1 in mice. The polysaccharides could significantly promote lymphocytes proliferation and IFN-γ secretion. The contents of CD4 +, CD8 + and CD3 +, the ratio of CD3 + and CD19+ (CD3+/CD19+) were promoted in the polysaccharides groups. Conclusion These polysaccharides could induce significant immune response against H1N1 in mice without observable toxicity. They should be further evaluated as a useful adduvant candidate for H1N1 influenza virus split vaccine.