Effects of iptakalim on renal injury induced by lipopolysaccharide, oleic acid and diethylene glycol / 国际药学研究杂志

ABSTRACT

Objective: To observe the effects of iptakalim on renal injury induced by lipopolysaccharide (LPS), oleic acid and diethylene glycol(DEG). Methods: By njection of 2% ead acetate and 1 μg (1 ml/kg) LPS to rats' femoral vein, 4 h later the experimental models of renal injury induced by LPS have been developed. By injection of 0.15 ml/kg oleic acid to rats' left renal artery, 24h later the experimental models of renal injury induced by oleic acid have been developed. Iptakalim was orally gavaged at the doses of 1,3,9 mg/(kg · d) for 3 d and 1 h before injury. The experimental models of renal injury have been developed by injection of DEG 10 g/kg to mice's peritoneal cavity, then iptakalim was orally gavaged at the doses of 1,3,9 mg/(kg·d) for 6 d. After the experimental models have been set up, observe the serum levels of creatinine(Cr), blood urea nitrogen(BUN) and pathological changes in renal tissue, for the further evaluation of renal function. Results: (1) In rats with the shock induced by LPS, significantly increased serum evels of Cr and BUN were found. Renal cortex of injury rats showed obviously glomerulus microthrombi, tubular cell swelling, necrosis, congestion and cast. Pretreatment of iptakalim at the dose of 9 mg/kg showed improved renal dysfunction and pathological changes in renal tissue. (2) In rats with the renal injury induced by oleic acid, significantly increased serum levels of Cr and BUN were found. Renal cortex of renal injury rats showed obviously glomerulus endothelial cell necrosis, tubular cell congestion and cell cast. Iptakalim had no effect on damaged renal function and the morphological changes in renal tissue. (3) In mice with the renal injury induced by 10 g/kg of DEG, significantly increased serum evels of Cr were found. Iptakalim at the doses of 9 mg/kg decreased serum evels of Cr to normal level. Conclusion: Iptakalim does not fit for individuals of renal damage caused by LPS or oleic acid. The protective effects of iptakalim against renal damaged by DEG need to be further investigated.