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Genetic diversity analysis on cultivar and wild population of Rehmannia glutinosa based on ISSR markers / 中草药
Chinese Traditional and Herbal Drugs ; (24): 6054-6061, 2020.
Article in Chinese | WPRIM | ID: wpr-846025
ABSTRACT

Objective:

To study the genetic diversity and genetic relationship of cultivar and wild population of Rehmannia glutinosa and its relative species by ISSR molecular marker technique, and provide the reference for R. glutinosa germplasm protection and breeding.

Methods:

106 samples including cultivar and wild population of R. glutinosa and its relative species were studied by ISSR-PCR markers. Nei's genetic diversity index (H) and other parameters of genetic information were calculated by POPGEN 32, and a cluster dendrogram of different samples was established based on the unweighted pair-group method with arithmetic mean (UPGMA) by NTSYS-pc software.

Results:

Seven ISSR primers generated 85 loci of which 83 loci were polymorphic. The percentage of polymorphie bands (PPB) of all samples was 97.65%. Nei's genetic diversity index (H) and Shannon's information index (I) were 0.2659 and 0.4125. The percentage of polymorphie bands (PPB) of cultivar of R. glutinosa was 30.59%. The percentage of polymorphie bands (PPB) of wild population of R.glutinosa in Henan province was 83.53%. In the cluster dendrogram, all samples were clustered into seven groups at the level of Genetic similarity coefficient (GS) 0.67.

Conclusion:

The results of ISSR analysis revealed that the level of genetic diversity between wild populations of R. glutinosa was higher than that within cultivar populations of R. glutinosa. The genetic diversity among wild populations of R. glutinosa in Henan province was higher than other region, which was consistent with authentic producing areas of R. glutinosa in this area. The relationships of wild population of R. glutinosa had no obvious correlation with their geographical distribution pattern.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2020 Type: Article