Establishment of HPLC fingerprint and determination of nine components in Wenjing Decoction / 中草药

ABSTRACT

Objective: To establish the HPLC fingerprint and simultaneously determinate nine components of the standard decoction of Wenjing Decoction, so as to provide reference for the quality control of Wenjing Decoction of classical prescriptions. Methods: Fingerprints of 15 batches of the standard decoction of Wenjing Decoction were determined by HPLC-PDA, and the control fingerprint was established. All samples were analyzed by Kromasil C18 chromatographic column (250 mm × 4.6 mm, 5 μm) maintained at 25 ℃, and eluted with acetonitrile-0.1% phosphoric acid at the flow rate of 0.8 mL/min, and the detection wavelength was 220, 280, 320 and 380 nm respectively. Combined with cluster analysis (CA), principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA), the quality of 15 batches of Wenjing Decoction was analyzed. At the same time, the contents of nine active components were determined. Results: The similarity of 15 batches of standard decoction of Wenjing Decoction was between 0.902 and 0.992, and a total of 18 common peaks were identified and nine of them (2-gallic acid, 5-paeoniflorin, 7-liquiritin, 8-ferulic acid, 9-isoliquiritin apioside, 11-isoliquiritin, 14-cinnamaldehyde, 15-ammonium glycyrrhetate, 16-paeonol) were quantitative analyzed. CA, PCA and PLS-DA were used to classify the 15 batches of samples into two groups. The results of quantitative analysis were good, and the recovery rate of nine components was 94.91%-108.16%. The content of gallic acid, paeoniflorin, iquiritin, ferulic acid, isoliquiritin apioside, isoliquiritin, cinnamaldehyde, ammonium glycyrrhetate, paeonol in 15 batches of samples were in the range of 10.7-31.3, 95.8-228.4, 18.6-62.4, 3.3-8.3, 4.8-18.7, 2.8-10.6, 13.7-108.2, 83.9-292.3, and 31.1-125.5 mg/g, respectively. Conclusion: The HPLC fingerprint combined with the simultaneous determination of multicomponent analysis method eatablished in this experiment are stable and reliable, which can provide the theoretical guidance for the quality evaluation of Wenjing Decoction and its compound preparations.