Chemical constituents of ethyl acetate extracts from root bark of Celastrus monospermus / 中草药

ABSTRACT

Objective: To study the chemical constituents of ethyl acetate extracts from the root bark of Celastrusmonospermus. Methods: The compounds were separated and purified by repeated column chromatographic methods including silica gel, ODS and Sephadex LH-20, and the chemical structures of compounds were determined by spectral data analyses. Results: Twelve compounds were obtained and identified as polpunonic acid (1), p-hydroxybenzoic acid (2), 4-hydroxy-3-methoxybenzoic acid (3), salaspermic acid (4), 3-oxo-2β-hydroxyfriedelan-29-oic acid (5), celastrol (6), 3α,22β-dihydroxy-olean-12-en-29-oic acid (7), orthosphenic acid (8), 21-oxo-2α,3α,22β-trihydroxy-29-nor-friedelan-24,2â-lactone (9), 2,3-dioxo-6α,10-dihydroxy-24-nor-friedelan-4,7-dien-29-oic acid (10), 2α,3β,19α,23-tetrahydroxyolean-12-en-28-oic acid (11) and 2α,3β-dihydroxyolean-12-en-23,28,30-trioic acid (12). Conclusion: Compounds 1, 3, 7, 10-12are obtained from this plant for the first time, and compounds 3, 11, 12 of them were separated from the genus Celastrus for the first time.