Research on grade evaluation method of Paeoniae Radix Rubra based on correlation of "biological activity-quality marker" / 中草药

ABSTRACT

Objective: A logistic regression model for grade evaluation of Paeoniae Radix Rubra medicinal slices was constructed based on the quality control idea of traditional Chinese medicines that "ingredients reflect activity and activity expresses effect". Methods: Q-marker content of paeoniflorin was tested by ultra-high performance liquid chromatography (UPLC). Anticoagulation valence, inhibition rate of hydroxyl radical and DPPH clearance rate were used as evaluation indexes of biological activity. Correlations between paeoniflorin content in different batches and bioactivity indexes were analyzed by the logistic algorithm. Finally, a "principal component analysis-Logistic regression" model for grade evaluation of Paeoniae Radix Rubra was constructed. Results: According to grade evaluation results, the grade probability of different batches of Paeoniae Radix Rubra was higher than 95%. Among 16 batches of Paeoniae Radix Rubra, 15 batches were evaluated excellent, good and moderate (five for each), and one batch was evaluated poor. Conclusion: A new grade evaluation method for Paeoniae Radix Rubra medicinal slices is constructed preliminarily. It is applicable to quality evaluation of Paeoniae Radix Rubra medicinal slices.