Application of fingerprint combined with QAMS in quality evaluation of Aurantii Immaturus Fructus / 中草药

ABSTRACT

Objective: This paper aims to provide the experimental foundation for quality control of Aurantii Immaturus Fructus from different habitats by analyzing the multi-component qualitatively and quantitatively. Methods: Fingerprint pattern and QAMS were combined in this experiment, and HPLC analysis was performed on a Waters X-Brige C18 (200 mm × 4.6 mm, 5 μm) column with mobile phase composed of acetonitrile (A) -0.1% methanoic acid solution at a flow rate of 1.0 mL/min in gradient elution mode. The column temperature was maintained at 30 ℃, the injection volume was 10 μL, and the detection wavelengths were set at 283 and 330 nm. Results: The HPLC-UV fingerprint of Aurantii Immaturus Fructus from different habitats was established with 21 common peaks, 9 of them were identified as narirutin, naringin, hesperidin, neohesperidin, naringenin, hesperetin, sinensetin, nobiletin and tangeretin, and the similarity of 23 batches of samples were greater than 0.9, CA and PCA show the classification is consistent with the habitats distribution of the medicine. Then, the QAMS result showed some differences with neohesperidin to be the internal standard. Conclusion: From the perspective of composition, there are some differences in the quality of Aurantii Immaturus Fructus from different habitats, and the method of QAMS is accurate, efficient and feasible, which has obvious advantages than standard curve method and external standard method. In a word, it can be applied to quality evaluation and provides the experimental foundation for quality control of Aurantii Immaturus Fructus from different habitats.