Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice
Experimental & Molecular Medicine
;
: 495-504, 2008.
Article
in English
| WPRIM
| ID: wpr-84653
ABSTRACT
Exposure to light can induce photoreceptor cell death and exacerbate retinal degeneration. In this study, mice with genetic knockout of several genes, including rhodopsin kinase (Rhok-/-), arrestin (Sag-/-), transducin (Gnat1-/-), c-Fos (c-Fos-/-) and arrestin/transducin (Sag-/-/Gnat1-/-), were examined. We measured the expression levels of thousands of genes in order to investigate their roles in phototransduction signaling in light-induced retinal degeneration using DNA microarray technology and then further explored the gene network using pathway analysis tools. Several cascades of gene components were induced or inhibited as a result of corresponding gene knockout under specific light conditions. Transducin deletion blocked the apoptotic signaling induced by exposure to low light conditions, and it did not require c-Fos/AP-1. Deletion of c-Fos blocked the apoptotic signaling induced by exposure to high intensity light. In the present study, we identified many gene transcripts that are essential for the initiation of light-induced rod degeneration and proposed several important networks that are involved in pro- and anti-apoptotic signaling. We also demonstrated the different cascades of gene components that participate in apoptotic signaling under specific light conditions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retina
/
Retinal Degeneration
/
Transducin
/
Genes, fos
/
Apoptosis
/
Mice, Knockout
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
GTP-Binding Protein alpha Subunits
/
G-Protein-Coupled Receptor Kinase 1
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2008
Type:
Article
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