Mechanism of matrine inhibiting proliferation of cervical squamous carcinoma cells by up-regulating LncRNA BDNF-AS / 中草药

ABSTRACT

Objective: To investigate the mechanism of matrine inhibiting the proliferation of cervical squamous cancer cells. Methods: CCK-8 method was used to detect the effect of matrine on the survival rate of SiHa and C33A cells, and flow cytometry was used to detect cell cycle. High throughput sequencing technology was used to screen the differentially expressed genes between control cells and cells being treated with matrine. qRT-PCR and Western blotting method were used to detect the expression of BDNF-AS and BDNF in cervical squamous cancer cells and allograft tumor tissue. A total of 32 cases of cervical squamous cell carcinoma tissues were collected in the Second People’s Hospital of Nanyang from March 2013 to December 2016. qRT-PCR was used to detect the expression of BDNF-AS in cervical squamous cell carcinoma tissues of these cases. Results: Matrine significantly inhibited the proliferation of cervical squamous carcinoma cells. A total of 924 differentially expressed genes were screened out from cervical squamous carcinoma cells before and after being treated with matrine, 637 (68.9%) were up-regulated while 287 (31.1%) were down-regulated. Matrine up-regulated the expression of BDNF-AS. The expression of BDNF-AS was negative correlated with the degree of pathological differentiation and clinical stage (P < 0.05). BDNF-AS expression in tissues was associated with survival of patients with cervical squamous cell carcinoma. Multivariate analyses suggested the expression of BDNF-AS was served as an independent predictor for overall survival. Conclusion: BDNF-AS may be a tumor suppressor in the tumorigenesis and tumor progression of cervical squamous cell carcinoma. Matrine may inhibit the proliferation of cervical squamous carcinoma cells by up-regulating the expression of BDNF-AS.