Simultaneous determination of eight active components in Qingfei Decoction by HPLC and its fingerprint analysis / 中草药

ABSTRACT

Objective: To simultaneously determinate the content of chlorogenic acid, amygdalin, gardenoside, hesperidin, baicalin, wogonoside, ammonium glycyrrhizate and schisandrin in Qingfei Decoction by HPLC-VWD, and establish the fingerprint of Qingfei Decoction. The results can be applied to characterize and distinguish commercially available Qingfei Decoction preparations from different manufacturers. Methods: Agilent Technologies Zorbax SB-C18 (250 mm × 4.6 mm, 3.5 μm) column was used, with acetonitrile (A)-0.1% phosphoric acid aqueous solution (B) as the mobile phase for gradient elution, column temperature of 35 ℃, flow rate of 0.6 mL/min, injection volume of 10 μL. The content of the indicator components and the fingerprints were simultaneously assayed by the above method. Fingerprint similarity evaluation, heat map cluster analysis and other methods were used to characterize and distinguish the commercially available Qingfei Decoction preparations from different sources. Results: The specificity, linear relationship (r2 > 0.999 8), precision (RSD ≤ 1.85%, n = 6), repeatability (RSD ≤ 1.82%, n = 6), stability (RSD ≤ 1.49%, 48 h ) of eight components were good; The recovery rate of the sample was in the range of (93.19 ± 1.93)% to (102.36 ± 4.17)% (n = 6). The results of content determination showed that the daily dosage of the indicator ingredients in the marketed Qingfei Decoction preparations fluctuated greatly in different manufacturers, such as baicalin, which fluctuated from 59.85 to 224.05 mg, but the fluctuation was not obvious in different dosage forms of the same manufacturer, for example, the content of baicalin in the two preparations of KPC Herbs was 140.00 to 142.47 mg. At the same time, the similarity calculation results of fingerprints were all greater than 0.945, which indicated that the fingerprints of Qingfei Decoction from different sources were highly similar. Conclusion: The method is accurate and reliable with good repeatability. It can be used to determine the content of eight components in the commercial Qingfei Decoction preparations and establish the fingerprint of Qingfei Decoction. The commercial Qingfei Decoction preparations can be characterized by heat map clustering analysis of the daily dosage of the eight components and the fingerprint similarity evaluation to distinguish the commercially available preparations of Qingfei Decoction from different sources.