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Qualitative and quantitative analysis of characteristic chemical constituents in wine-processed Corni Fructus by chemometric and UPLC-PDA / 中草药
Chinese Traditional and Herbal Drugs ; (24): 1294-1301, 2020.
Article in Chinese | WPRIM | ID: wpr-846568
ABSTRACT

Objective:

To screen the differential ingredients between crude and wine-processed Corni Fructus and determin their content.

Methods:

An integrated strategy using ultra performance liquid chromatography coupled with tandem quadrupole time-of- flight mass spectrometry (UPLC-Q-TOF/MS) and the chemometric approach was applied to compare the global chemical profile of crude and wine-processed Corni Fructus. Then, the main differential ingredients were quantified by UPLC-PDA.

Results:

The chemical profiling of wine-processed Corni Fructus was significantly different. Ten compounds could be considered as characteristic chemical markers for distinguishing crude and wine-processed Corni Fructus, including 5-hydroxymethyl furfuraldehyde (5-HMF), gallic acid, protocatechuic acid, morroniside, loganic acid, sweroside, cornin, dihydroquercetin, loganin and cornoside. A new UPLC-PDA quantitative method for analyzing simultaneously the above ten compounds in wine-processed Corni Fructus was established. The results of methodology investigation showed that the ten components were well linear within the investigation range (r ≥ 0.999 7). Compared with the crude Corni Fructus, the content of seven components were increased, including gallic acid, 5-HMF, loganin, morroniside, cornin, sweroside and dihydroquercetin, and the other three components in wine-processed Corni Fructus were decreased.

Conclusion:

The differential ingredients obtained by chemometric-based approach can be used to distinguish crude and wine-processed Corni Fructus. The determination method of wine-processed Corni Fructus established is accurate and reliable, which can be used for the quality control of Corni Fructus.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Qualitative research Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Qualitative research Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2020 Type: Article