Cloning and expression analysis of iridoid synthase gene in Centranthera grandiflora / 中草药

ABSTRACT

Objective: To clone the CgIS gene encoding iridoid synthase from Centranthera grandiflora and conduct its expression analysis. Methods: Based on the sole sequence of CgIS gene in root, stem and leaf transcriptome of C. grandiflora, a CgIS gene was cloned from young leaves of C. grandiflora by RT-PCR technique, and its tissue-specific expressions were also performed. Results: The CgIS gene (GenBank accession number MH794270) had a length of 1 185 bp coding for 394 amino acids, and the relative molecular weight of CgIS protein was 44 670 with its theoretical pI of 6.17. CgIS protein belonged to the member of P5βR (progesterone 5β-reductase) family, and might localize in cytoplasm. CgIS protein was a hydrophilic stable protein without signal peptide, and composed of mainly α-helix (40.61%) and coil (46.70%). The SDR (short-chain dehydrogenase/reductase) and P5βR conserved domains were existed in CgIS protein. CgIS protein was close to SiIS protein of Sesamum indicum. CgIS gene was primarily expressed in leaves. Conclusion: The CgIS gene is cloned, and its expressions are also analyzed, which will pave a way for further studies on function of CgIS gene and biosynthetic pathway of iridoids.