Effects of ethanol extract of Songjie Fang on expression of tyrosine kinase and phosphatase-related molecules in TCR signaling pathway in rats with rheumatoid arthritis / 中草药

ABSTRACT

Objective: To investigate the effectof ethanol extract of Songjie Fang (SJF) effect on the expression of tyrosine kinase and phosphatase-related molecules in the T cell antigen receptor (TCR) signaling pathway in rheumatoid arthritis rats and its mechanism. Methods: The rheumatoid arthritis (RA) rat model was administered SJF for 2 weeks. Then the content of tyrosine kinase (Lck, Fyn, ZAP70) and tyrosine phosphatase (CD45) in joint fluids was detected by enzyme-linked immunosorbent assay (ELISA). To observe the histopathological changes of the ankle joints, and the expressions of Lck, Fyn, ZAP70 and CD45 in the synovial tissue were detected by immunohistochemistry. Results: The ethanol extract of SJF significantly reduced the content of Lck, Fyn, ZAP70 and CD45 in joint fluid, inhibited the infiltration of inflammatory cells and hyperplasia in the synovial tissue. Meanwhile, down-regulated the expression of Lck, Fyn, ZAP70 and CD45 in synovial tissue significantly. Conclusion: The mechanism of anti-inflammation affected by ethanol extract of SFJ may be related to inhibiting the tyrosine kinase and phosphatase-related molecules in the TCR signaling pathway.