Determination of triglycerides and fatty acids in Coicis Semen from different areas and its quality evaluation / 中草药

ABSTRACT

Objective: To determine the seven kinds of triglycerides and two kinds of unsaturated fatty acids in Coicis Semen in order to evaluate the quality of Coicis Semen in different producing areas. Methods: HPLC-ELSD method was used to determine the content of seven triglycerides in Coicis Semen. The HPLC-UV method was established to determine the content of oleic acid and linoleic acid. The cluster analysis method was used for quality evaluation. Results: The content of trilinolein, 1,2-dilinoleic acid-3-olein, 1,2-dilinoleic acid-3-palmitin, 1,2-dioleic acid-3-linolein, 1-palmitic acid-2 oleic acid-3-linolein, triolein, 1,2-dioleic acid-3-palmitin were 0.31%-0.83%, 0.81%-1.81%, 0.38%-0.95%, 1.19%-2.39%, 0.67%-1.58%, 0.71%-1.55%, 0.44%-1.13%, respectively. The content of oleic acid and linoleic acid in 14 batches of Coicis Semen were 0.45%-0.72% and 0.37%-0.53%, respectively. Adopting triglyceride and fatty acid as variables, the samples can be grouped into three categories. Conclusion: Some differences existed in content of triglyceride and fatty acid in Coicis Semen from different areas, but the clustering results did not find the correlation between its content and origin. The determination of multi-components comprehensively reflected the quality of Coicis Semen.