Thermosensitive chitosan-collagen composite hydrogel loaded with basic fibroblast growth factor retards ventricular remodeling after myocardial infarction in mice / 中国组织工程研究

ABSTRACT

BACKGROUND: Chitosan or collagen alone can improve the microvessel density in myocardial infarction area and participate in the short-term myocardial repair after myocardial infarction. Based on this research group, thermosensitive chitosan-collagen composite hydrogel was prepared, which confirmed that it had good physical and chemical properties and was suitable for myocardial tissue engineering scaffold materials. OBJECTIVE: To prepare the chitosan-collagen composite hydrogel loaded with basic fibroblast growth factor, and to analyze its physical and chemical properties, biocompatibility and prognosis in vivo after treatment of myocardial infarction in mice. METHODS: (1) Basic fibroblast growth factor was loaded in chitosan-collagen composite hydrogel and chitosan hydrogel separately, and the release efficiency of basic fibroblast growth factor in hydrogels was detected. (2) H9C2 cardiomyocytes were seeded in two hydrogels loaded with basic fibroblast growth factor, and cell proliferation was detected by CCK8 assay. (3) C57BL/6J mice model of myocardial infarction was established and randomly divided into three groups. The blank group was injected PBS into the myocardial infarction area. The control group was injected with chitosan hydrogel loaded with basic fibroblast growth factor. The experimental group was injected with chitosan-collagen composite hydrogel loaded with basic fibroblast growth factor. Echocardiography and myocardial histology were performed 28 days after operation. The experiment was approved by Ethics Committee of Shanxi Medical University. RESULTS AND CONCLUSION: (1) Compared with chitosan hydrogel, chitosan-collagen composite hydrogel could promote the release of basic fibroblast growth factor in gel. (2) Compared with the chitosan hydrogel loaded with basic fibroblast growth factor, the chitosan-collagen composite hydrogel loaded with basic fibroblast growth factor could promote the proliferation of cardiomyocytes. (3) Echocardiography showed that the left ventricular ejection fraction and the shortening rate of left ventricular short axis in the experimental group and the control group were higher than those in the blank group (P < 0.05). Left ventricular ejection fraction and the shortening rate of left ventricular short axis in the experimental group were higher than those in the control group (P < 0.05). (4) The myocardial histological observation showed that the infarct area of the experimental group and the control group was smaller than that of the blank group (P < 0.05). The infarct area wall thickness of the experimental group and the control group was larger than that of the blank group (P < 0.05). The infarct area of the experimental group was smaller than that of the control group (P < 0.05), and the infarct area wall thickness of the experimental group was larger than that of the control group (P < 0.05). (5) The results showed that the chitosan-collagen composite hydrogel loaded with basic fibroblast growth factor had good biocompatibility, and animal transplantation could significantly reduce the ventricular remodeling after myocardial infarction.