Expression of tissue transgiutaminase 2 in differentiation of nasal mucosal mesenchymal stem cells / 中国组织工程研究

ABSTRACT

BACKGROUND: Ectodermal mesenchymal stem cells can differentiate into other types of cells in a specific external environment. The expression of some specific proteins during differentiation may directly affect the direction of their fate. OBJECTIVE: To investigate the expression of tissue transgiutaminase 2 in rat nasal mucosal mesenchymal stem cells during osteoblast differentiation and neuron-like differentiation. METHODS: Rat nasal mucosal mesenchymal stem cells were isolated and cultured by tissue adherence method, and differentiated into neuroblast-like cells and osteoblasts. Immunofluorescence was used to detect the expression of neuro-related proteins GAP-43, TUBB3 and bone-associated proteins OCN and COLI at different time points. Western blot assay was used to detect neuro-related proteins GAP-43, TUBB3, bone-associated protein OCN and COLI, neurotrophic factor NT-3, BDNF, extracellular matrix FN, LN, and tissue transgiutaminase 2. RESULTS AND CONCLUSION: (1) After 3 days of neurogenic induction, immunofluorescence showed that GAP-43 and TUBB3 expressed positively. At 7 days after osteogenic induction, COLI and OCN were positive under immunofluorescence staining. (2) At 3, 5, 7 days after neurogenic induction, the expression levels of nerve cell-associated proteins GAP-43, TUBB3 and tissue transgiutaminase 2 were increased with time detected by western blot assay. At 7 and 14 days after osteogenic induction, the levels of osteoblast-associated proteins COLI, OCN and tissue transgiutaminase 2 were increased with time. (3) At 7 days after neurogenic induction, western blot assay showed that the levels of NT-3, BDNF and tissue transgiutaminase 2 were higher than those in the non-induction group. At 14 days after osteogenic induction, the expression levels of FN, LN and tissue transgiutaminase 2 in the extracellular matrix were higher than those in the non-induction group. (4) To conclude, tissue transgiutaminase 2 may regulate the differentiation of nasal mucosal mesenchymal stem cells into neuron-like cells and osteoblasts. Key words nasal mucosal mesenchymal stem cells; osteoblasts; neural-like cells; tissue transgiutaminase 2 Funding: the National Natural Science Foundation of China, No. 81571830 (to ZZJ); the Clinical Medicine Science and Technology Development Project of Jiangsu University, No. JLY20180040 (to ZZJ).