Heat-shock endothelial cells induce differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells / 中国组织工程研究

ABSTRACT

BACKGROUND: Current research on the differentiation of mesenchymal stem cells into endothelial cells mainly focuses on the induction of cytokines or co-culture of two types of cells. Research has been not yet reported on heat shock-treated endothelial cells inducing the differentiation of mesenchymal stem cells into endothelial cells. OBJECTIVE: To investigate the ability of heat shock-treated endothelial cells to induce bone marrow mesenchymal stem cells to differentiate into vascular endothelial cells, and to explore the angiogenic capacity of induced bone marrow mesenchymal stem cells. METHODS: Human bone marrow mesenchymal stem cells were co-cultured with heat shock-treated human umbilical vein endothelial cells in a non-contact manner. The expression of CD31, CD144, VEGFR2 and vWF in the bone marrow mesenchymal stem cells was detected by flow cytometry at 14 days after co-culture. The immunofluorescence of the cells was further verified. In vivo angiogenesis test was detected using hematoxylin-eosin staining in nude mice undergoing subcutaneous transplantation of induced and non-induced bone marrow mesenchymal stem cells. Matrigel angiogenesis test was applied to observe the angiogenic ability of induced and non-induced bone marrow mesenchymal stem cells. RESULTS AND CONCLUSION: After coculture, bone marrow mesenchymal stem cells had paving-stone like structure. Flow cytometry and cellular immunofluorescence results showed an increase in the expression of VEGFR2, vWF, CD31 and CD144 after co-culture. The hematoxylin-eosin staining of the graft showed that the induced bone marrow mesenchymal stem cells arranged in a regular manner and had an angiogenic tendency. The Matrigel angiogenesis test showed that the angiogenic ability of bone marrow mesenchymal stem cells was increased after induction compared with the control group. These findings indicate that co-culture with heat shock-treated human umbilical vein endothelial cells can promote the differentiation of mesenchymal stem cells into endothelial cells, and the specific phenotype of endothelial cells is obviously transformed, which has a certain tendency of angiogenesis.