Effect of icaritin on ferroptosis of bone marrow mesenchymal stem cells and their differentiation into cardiomyocytes / 中国组织工程研究

ABSTRACT

BACKGROUND: Iron accumulation or iron overload is closely related to the proliferation and apoptosis of bone marrow mesenchymal stem cells, which may play an important role in the pathological process of cardiovascular diseases. OBJECTIVE: To investigate the regulatory effect of icariin on ferroptosis of bone marrow mesenchymal stem cells and its differentiation into cardiomyocytes. METHODS: Taking bone marrow mesenchymal stem cells as the research object, the blank group was cultured for 14 days. The model group was treated with 0.4 μmol/L Erastin for 14 days to establish ferroptosis model. The treatment group was treated with 80 nmol/L icariin and 0.4 μmol/L Erastin for 14 days. CCK-8 method was used to detect the proliferation of cells in each group, and the levels of malondialdehyde and superoxide dismutase were detected by related kits. Western blot assay was used to detect the expression of TFR1, FPN1, Bcl-2 and Bax protein. RT-PCR was used to detect the mRNA expression of GATA4 and cTnT. RESULTS AND CONCLUSION: (1) Compared with the model group, the cell proliferation ability of the treatment group was significantly improved (P < 0.05). (2) Compared with the model group, the level of malondialdehyde in the lysate of bone marrow mesenchymal stem cells in the treatment group was significantly lower (P < 0.05), while the level of superoxide dismutase was significantly increased (P < 0.05). (3) Compared with the model group, TFR1 protein expression was decreased (P < 0.05), FPN1 protein expression was increased (P < 0.05), and Bcl-2/Bax protein expression ratio was increased significantly (P < 0.05) in the treatment group. (4) Compared with the model group, the expression of GATA4 and cTnT mRNA was significantly increased in the treatment group (P < 0.05). (5) The results showed that icariin could inhibit the ferroptosis of bone marrow mesenchymal stem cells and improve the ability of bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells.