Effect of peroxiredoxin 6 on proliferation and differentiation of bone marrow mesenchymal stem cells into neural lineage in vitro / 中国组织工程研究

ABSTRACT

BACKGROUND: It has been found that many substances can promote the differentiation of bone marrow mesenchymal stem cells into neural lineage in vitro, but the effect of peroxiredoxin 6 on bone marrow mesenchymal stem cells has not been studied. OBJECTIVE: To explore the effects of peroxiredoxin 6 on the proliferation of rat bone marrow mesenchymal stem cells and the ability to differentiate into neural lineages in vitro. METHODS: Bone marrow mesenchymal stem cells of Sprague-Dawley rats were cultured and passaged by whole bone marrow adherent culture in vitro into passage 3. The cells were assigned to five groups PBS group, 1, 10, 100 μg/L and 1 mg/L peroxiredoxin 6 groups. CCK-8 assay was used to measure the absorbance at 450 nm of each group of cells for 9 consecutive days. Cell growth curves were drawn. Enzyme linked immunosorbent assay was utilized to measure absorbance at 450 nm in platform period. A relatively optimal concentration of peroxiredoxin 6 was selected to induce bone marrow mesenchymal stem cells to differentiate into neural lineages. PBS served as the control group. After 7 days of differentiation, immunofluorescence staining and western blot assay were used to detect the expression of neuronal marker protein NSE and glial cell marker protein GFAP. RESULTS AND CONCLUSION: (1) 1, 10 and 100 μg/L peroxiredoxin 6 groups complied with the general growth rule of bone marrow mesenchymal stem cells, and 10 μg/L was the optimal mass concentration. (2) NSE and GFAP immunofluorescence staining showed positive reaction after peroxiredoxin 6 induction; NSE and GFAP expression levels were higher than those in the control group. (3) The results of in vitro experiments show that the proper concentration of peroxiredoxin 6 can promote the proliferation of bone marrow mesenchymal stem cells and induce differentiation into neural lineage.