Preparation method of acellular matrix and its application in tissue engineering of parotid gland / 中国组织工程研究

ABSTRACT

BACKGROUND: Bioscaffolds composed of acellular matrix are widely used In animal and clinical research to repair and reconstruct tissues and organs, but all decellularlzatlon methods will destroy matrix structure and function to some extent. OBJECTIVE: To review the preparation methods, advantages and disadvantages of acellular matrix and its application in the study of parotid gland and other tissue engineering. METHODS: A computer-based search of CNKI, CBM, PubMed and Elsevier databases was performed to retrieve related articles published from 2008 to 2019. The search term were “decellular matrix, preparation method, parotid gland, tissue engineering, regeneration, decellularizatlon, preparation method, parotid gland tissue engineering” In Chinese and English, respectively. Seventy-four eligible articles were Included. RESULTS AND CONCLUSION: Acellular matrix preparation methods of most tissues and organs require chemical, biological (enzyme), physical methods and the their combination, which depend on the thickness, compositions and nature. Although not all decellularization methods can remove cellular components from tissues and organs, tissues and organs that completely remove cells have the advantage of remodeling tissue specificity, providing a favorable microenvironment for the proliferation and differentiation of inoculated cells. Due to the complex structure of the parotid gland and the challenges of tissue engineering In clinical applications, clinical transplantation In patients Is limited. In vivo studies In this field are limited to animals, and applications based on submandibular gland acellular matrix bioscaffold materials are expected to be a favorable source of organizational parotid glands.