Toxic effects of different-concentration isoniazid on newborn rat osteoblasts in vitro / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 1162-1167, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-847960
ABSTRACT
BACKGROUND:
As a first-line antituberculosis drug, isoniazide exerts strong sterilization effect on the Tubercle bacillus inside and outside the cells. But the toxic effect of isoniazide on osteoblasts is never reported.OBJECTIVE:
To investigate the effect of different concentrations of isoniazide on the proliferation and differentiation of osteoblasts from newborn Sprague-Dawley rats.METHODS:
Isoniazide treatment at 0, 10, 20, 30, 40, 50, 60, and 100 mg/L was applied on passage 3 osteoblasts from newborn Sprague-Dawley rats. Then, osteoblast proliferation and differentiation was detected using cell counting kit-8 assay, alkaline phosphatase activity kit and immunohistochemical staining. RESULTS ANDCONCLUSION:
Compared with the control group, the proliferation of osteoblasts was significantly inhibited when the isoniazide concentration was 30 mg/L. In addition, increasing isoniazide concentrations inhibited alkaline phosphatase activity and type I collagen expression. To conclude, isoniazide at exorbitant concentration exerts adverse effects on the proliferation and differentiation of osteoblasts.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2020
Type:
Article
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