Changes of mitochondrial respiratory function and phb1 expression in rat skeletal muscle after moderate-intensity training / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 1207-1212, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-847967
ABSTRACT
BACKGROUND:
Studies have shown that long-term moderate-intensity regular exercise can Improve the activity of mitochondrial respiratory chain complex enzymes In skeletal muscle cells, thereby Improving their power capability as well as the ability to resist fatigue.OBJECTIVE:
To explore the effect of moderate-intensity training on prohibitin (PHB) expression and mitochondrial respiratory function in rat skeletal muscle.METHODS:
Thirty-two healthy male Sprague-Dawley rats were randomly divided into two groups Quiet control group and moderate-intensity training group, each of 16 rats. In the moderate-intensity training group, the treadmill training was performed at a slope of 10° In the 1st week, running at 10 m/min, 10 minutes per day, 6 days per week; in the 2nd week, running at 15 m/min, increased from 10 minutes per day to 60 minutes per day with an increase of 10 minute per day, 6 days per week; in the 3rd to 8th week, running at 15 m/min, 60 minutes per day, 6 days per week for a total of 8 weeks. Rats were sacrificed at 48 hours after the final experiment, and mitochondria were extracted from skeletal muscle samples. Mitochondrial respiration control rate, adenosine triphosphate content, reactive oxygen species level, complex V activity and PHB1 protein expression were measured. RESULTS ANDCONCLUSION:
Compared with the quiet control group, in the moderate-intensity training group, mitochondrial respiration control rate in the skeletal muscle increased significantly (P < 0.001), adenosine triphosphate content increased significantly (P < 0.05), reactive oxygen species level decreased significantly (P < 0.001), the activity of complex V was significantly increased (P < 0.05) and PHB1 expression was significantly increased (P < 0.01). The correlation analysis showed that after 8 weeks of moderate-intensity training, the expression of PHB1 in the skeletal muscle of rats was positively correlated with adenosine triphosphate content and complex V activity, and negatively correlated with reactive oxygen species level. Therefore, moderate-intensity training improves mitochondrial oxidative phosphorylation, effectively maintains mitochondrial membrane structure and enhances mitochondrial respiratory function by increasing the expression of PHB1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2020
Type:
Article
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