Mechanism of hanshi bi granules in the treatment of ankylosing spondylitis based on network pharmacology / 中国组织工程研究

ABSTRACT

BACKGROUND: Hanshi Bi granule has been used to treat ankylosing spondylitis in the clinic, but the pharmacological mechanism B is still unclear. OBJECTIVE: To screen the compound and drug targets of Hanshi Bi granules based on network pharmacology, and construct a compound-target network to systematically investigate the pharmacological mechanism for treating ankylosing spondylitis. METHODS: The compounds and drug targets of Hanshi Bi granules were collected from TCMSP database. Ankylosing spondylitis targets were from DRUGBANK, GeneCards, Home-OMIM-NCBI, and PALM-IST databases. The obtained drug targets and disease targets were corrected by Uniprot database and common genes were obtained using Draw Venn Diagrams analysis tool. PPI analysis was performed on the common gene in combination with the STRING database and Cytoscape 3.6.1. GO enrichment analysis and KEGG pathway enrichment analysis were performed on the common gene by using Cytoscape 3.6.1 plugin ClueGO. RESULTS AND CONCLUSION: (1) Totally 69 active ingredients and 142 drug targets were obtained from TCMSP, and 595 ankylosing spondylitis targets were obtained using DRUGBANK, GeneCards, Home-OMIM-NCBI, and PALM-IST databases. (2) Using the Draw Venn Diagrams tool to analyze all targets, 39 common genes were obtained. Through PPI analysis, tumor necrosis factor, interieukin-6 and prostaglandin-in-peroxidase 2 were highly connected in the PPI network. (3) Through GO enrichment analysis, it mainly involved biological functions such as regulation of reactive oxygen metabolism, fatty acid metabolism, acute inflammatory reaction, neurotransmitter biosynthetic process, and metabolism of arachidonic acid. (4) Through KEGG pathway enrichment analysis, it mainly involved in AGE-RAGE signaling pathway, fluid shear stress and atherosclerosis, irtterieukin-17 signaling pathway, nuclear factor-KB signaling pathway, tumor necrosis factor signaling pathway, and Toll-like receptor signaling pathway. (5) This study preliminarily predicts the pharmacological mechanism underlying the treatment of ankylosing spondylitis with Hanshi Bi granules, and provides new ideas for secondary development of old drugs and experimental research.