Effects of cytokine-induced neutrophil chemoattractant 3 on the survival and proliferation of neural stem cells / 中国组织工程研究

ABSTRACT

BACKGROUND: Our previous study was the first to find that bone marrow stromal cells could secrete neutrophil chemokine-3. Moreover, the expression of cytokine-induced neutrophil chemoattractant 3 (CINC-3) could be up-regulated by 1. 5 times during the differentiation of neural stem cells into neurons regulated by bone marrow stromal cells. These results suggest that CINC-3 may promote the proliferation and differentiation of neural stem cells. OBJECTIVE: To observe the effect of CINC-3 on survival and proliferation of hippocampal neural stem cells in neonatal rats. METHODS: Hippocampal neural stem cells from neonatal Sprague-Dawley rats were isolated and cultured in vitro, and passage 3 neural stem cells were divided into control and CINC-3 groups. The survival rate of the cells was measured by MTS method, cell survival and proliferation were observed using cell growth curve and live/dead cell staining, and the expression of Nestin in neural stem cells was detected by real-time PCR and immunofluorescence staining for observation of neural stem cell proliferation. RESULTS AND CONCLUSION: (1) When the concentration of CINC-3 increased from 1 to 20 μg/L, the survival rate of neural stem cells increased gradually. When the concentration of CINC-3 was 10 μg/L, the survival rate of neural stem cells was the highest and the cell viability was the best (P < 0. 05). When the concentration of CINC-3 further increased to 20 μg/L, there was no significant difference in the survival rate of neural stem cells. (2) The positive rate of Nestin in the CINC-3 group was significantly higher than that in the control group (P < 0. 05). (3) The expression of Nestin mRNA in the CINC-3 group was significantly higher than that in the control group (P < 0. 05). These findings indicate CINC-3 can promote the survival and proliferation of hippocampal neural stem cells.