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Changes of bone mineral density and related factors during maxillary teeth occlusion after tooth extraction in diabetic mice / 中国组织工程研究
Chinese Journal of Tissue Engineering Research ; (53): 242-247, 2020.
Article in Chinese | WPRIM | ID: wpr-848091
ABSTRACT

BACKGROUND:

Defective dentition is a common oral disease, if it is not treated in time, there will be adverse effects such as tilting of the adjacent teeth and elongation of the jaws, causing occlusal disorder and interference, which will seriously affect the later repair. Especially in the diabetic patients with dentition loss, the impacts of diabetes on the occlusal elongation of the jaw teeth, and how osteonectin changes in this process, are still unclear.

OBJECTIVE:

To investigate the effect of diabetes on tooth occlusion and elongation in mice by establishing a model of the occlusion of the jaws in diabetic mice.

METHODS:

A diabetic model was established by intraperitoneal injection of streptozotocin in C57 BL/6J mice (purchased from the Animal Experimental Center of Shanxi Medical University). The mice were intraperitoneally injected with sodium citrate buffer. Thirty mice with successful modeling and control mice were removed, and the three right maxillary molars were removed to establish an experimental model of the extensional movement of the maxillary teeth. After 0, 3, 6, 9 and 12 days, the right jaw was taken. The bone mineral density was measured by micro-CT. The number of osteoclasts was counted by tartrate resistant acid phosphatase staining. The expression level of osteonectin was detected by RT-qPCR. The study was approved by the Ethical Committee of Shanxi Medical University. RESULTS AND

CONCLUSION:

(1) With the time increasing, the bone mineral density of the right mandible in the two groups was gradually increased. The bone mineral density in the diabetic group was significantly lower than that in the control group at 3, 6, 9 and 12 days after surgery (P < 0.05). (2) With the time increasing, the number of osteoclasts in the right mandible of both groups was gradually increased. The number of osteoclasts in the diabetic group was significantly lower than that in the control group at 3, 6, 9 and 12 days after surgery (P < 0.05). (3) The expression level of osteonectin mRNA in the right mandible of both groups was gradually increased. The expression level of osteonectin mRNA in the diabetic group was significantly higher than that in the control group at 0, 3,6, 9 and 12 days after surgery (P< 0.05). (4) These results indicate that diabetes can reduce the bone construction ability during the extensional movement and promote osteonectin mRNA expression.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2020 Type: Article