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Over-expression of WNT5B inhibits migration and invasion of human breast cancer MDA-MB-231 cells / 肿瘤
Tumor ; (12): 164-170, 2019.
Article in Chinese | WPRIM | ID: wpr-848267
ABSTRACT

Objective:

To investigate the effects of WNT5B over-expression on the invasion and migration of human breast cancer MDA-MB-231 cells, and to explore its possible mechanisms.

Methods:

After the recombinant vector pcDNA3.1-WNT5B+EGFP containing enhanced green fluorescent protein (EGFP) and WNT 5B genes was transfected into human breast cancer MDA-MB-231 cells, the expression levels of WNT5B mRNA and protein were examined by real-time fluorescent quantitative PCR and Western blotting, respectively. The abilities of cell migration and invasion were analyzed by wound-healing test and Transwell chamber assay, respectively. Furthermore, the expression levels of epithelial-mesenchymal transition (EMT)-related molecules N-cadherin, E-cadherin and Vimentin were detected by Western blotting.

Results:

Compared with the untransfection and the empty vector-transfection groups, the expression levels of WNT5B mRNA (P < 0.01) and protein (P < 0.05) in MDA-MB-231 cells transfected with the recombinant vector pcDNA3.1-WNT5B+EGFP were up-regulated. The abilities of migration (P < 0.05) and invasion (P < 0.01) of MDA-MB-231 cells after WNT5B over-expression were significantly decreased. The expression level of E-cadherin in MDAMB- 231 cells with WNT5B over-expression was up-regulated (P < 0.05), whereas the expression levels of N-cadherin and Vimentin were down-regulated (both P < 0.05).

Conclusion:

WNT5B over-expression can inhibit the migration and invasion of breast cancer MDA-MB-231 cells, which may be related to the regulation of EMT process.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Tumor Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Tumor Year: 2019 Type: Article