Inhibitory effect of estrogen receptors aptamer on proliferation of breast cancer MCF-7 cells / 肿瘤

ABSTRACT

Objective: To screen an estrogen receptor (ER) aptamer, and to investigate its inhibitory effect on the proliferation of breast cancer MCF-7 cells. Methods: ER protein was purified by affinity chromatography, and ER aptamer was screened through systematic evolution of ligands by exponential enrichment (SELEX). The binding affinity between ER protein and ER aptamer was analyzed by Scatchard method. The survival rate of MCF-7 cells treated with different concentrations of ER aptamer, tamoxifen and fulvestrant for 24, 48 and 72 h was detected by MTT method. The proliferation of MCF-7 cells treated with different concentrations of estradiol alone or in combination with ER aptamer, tamoxifen and fulvestrant was detected by MTT method. The survival rate of human normal primary mammary epithelial HMEC cells treated with different concentrations of ER aptamer was detected by MTT method. Results: The synthesized ER aptamer in vitro showed a high affinity for ER molecules. As compared with the untreated control group, ER aptamer at doses of 10, 100 and 1 000 μmol/L could inhibit the survival rate of MCF-7 cells to 72%, 66%, and 19%, respectively (all P 0.05). Conclusion: ER aptamer inhibits the proliferation of MCF-7 cells by selectively antagonizing ER. ER aptamer is a potential ER antagonist in the treatment of breast cancer.