Antitumor effect of osthole on human B-cell acute lymphoblastic leukemia 697 cells and its mechanism / 肿瘤
Tumor
;
(12): 91-98, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-848278
ABSTRACT
Objective:
To investigate the antitumor effect of osthole on human B-cell acute lymphoblastic leukemia (B-ALL) 697 cells and its possible mechanism.Methods:
After B-ALL 697 cells were treated with different concentrations (8, 16, 32, 64 and 128 μmol/L) of osthole, the inhibition rate of cell proliferation was detected by CCK-8 assay. After B-ALL 697 cells were treated with 8 and 32 μmol/L osthole, the apoptosis was detected by FCM, the expressions of apoptosis-associated molecule Bcl-2 and Bax were detected by real-time fluorescent quantitative PCR and Western blotting. After B-ALL 697 cells were treated with 8 and 32 μmol/L osthole alone or in combination with autophagy inhibitor 3-methyladenine (3-MA), the intracellular mean fluorescent intensity (MFI) was detected by FCM [stained by monodansylcadaverine (MDC)] to reflect the autophagy. The expressions of autophagy-associated molecule Beclin 1 mRNA and protein was detected by real-time fluorescent quantitative PCR and Western blotting, respectively.Results:
The proliferation of B-ALL 697 cells in 8, 16, 32, 64 or 128 μmol/L osthole treatment group was significantly inhibited in a dose-and time-dependent manner (all P 0.05).Conclusion:
Osthole inhibits the proliferation, and induces the apoptosis and autophagy of B-ALL 697 cells. The mechanism of promoting apoptosis may be related to the up-regulation of Bax expression and the down-regulation of Bcl-2 expression. Beclin 1 participates in the autophagy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tumor
Year:
2019
Type:
Article
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