Mechanisms of matrine inhibiting proliferation and migration of gastric cancer MGC-803 cells / 肿瘤

ABSTRACT

Objective: To investigate the molecular mechanisms of matrine (MAT) inhibiting the proliferation and migration of gastric cancer MGC-803 cells. Methods: MGC-803 cells were treated with different concentrations of MAT. MTT assay was used to test the proliferation activity of MGC-803 cells. The colony-forming assay was used to detect the colony formation ability of MGC-803 cells. Wound healing assay and Transwell chamber assay were used to detect the lateral and vertical migration abilities of MGC-803 cells, respectively. The cell cycle distribution was detected by FCM. The protein expression levels of epithelial-mesenchymal transition (EMT) markers and matrix metalloproteinase (MMP) family members (MMP2 and MMP9) in MGC-803 cells were detected by Western blotting. Results: MAT significantly inhibited the proliferation (P < 0.01), colony formation (P < 0.05) and lateral and vertical migration (both P < 0.01) of MGC-803 cells, and blocked the cell cycle at G0/G1 phase (P < 0.01). MAT significantly decreased the expression levels of Vimentin (P < 0.05), Snail (P < 0.01), MMP2 (P < 0.01) and MMP9 (P < 0.01) proteins, while the expression level of E-cadherin protein was up-regulated (P < 0.05). Conclusion: MAT may effectively suppress the proliferation and migration of gastric cancer MGC-803 cells, and block cell cycle at G0/G1 phase. The mechanism may be related to the regulation of EMT-related protein expression.