A novel TNS4 mutant in prostate cancer cells and its mechanism / 肿瘤
Tumor
;
(12): 1-9, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-848429
ABSTRACT
Objective:
To investigate the expression and mutation status of tensin family member 4 (TNS4) gene in various prostate cancer cell lines, and to explore its potential molecular mechanism.Methods:
The expression of TNS4 gene in several prostate cancer cell lines was detected by RT-PCR. The full-length cDNA of TNS4 gene was amplified for the restriction endonuclease hydrolysis and DNA sequencing analysis. Meantime, the recombinant plasmids carrying wild- or mutant-type TNS4 gene were constructed and transfected into prostate cancer PC-3 cells, then the effects of TNS4 over-expression on the mRNA and protein expressions as well as the localization of signal transducer and activator of transcription 1 (STAT1) were detected by RT-PCR, Western blotting and immunofluorescence microscopy, respectively.Results:
As compared with benign prostatic hyperplasia BPH1 cells, TNS4 gene was lowly expressed in prostate cancer cell lines DU145, LNCaP and 22Rv1 (all P 0.05). Additionally, the over-expression of mutant TNS4 significantly promoted nuclear translocation of STAT1 protein (P < 0.01).Conclusion:
TNS4 gene is lowly expressed in prostate cancer cell lines, and there is a novel TNS4 mutant (S143P). TNS4 over-expression can up-regulate the expression of STAT1 protein, furthermore the mutant TNS4 can alter the location of STAT1 protein.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tumor
Year:
2018
Type:
Article
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