The expression of Wnt2 in human breast cancer tissues and its effect on the invasion of breast cancer cells / 肿瘤

ABSTRACT

Objective: To investiget the expression of Wnt2 in human breast cancer tissues and its effect on the invasion of breast cancer cells. Methods: The serum Wnt2 content of patients with breast cancer and the healthy volunteers was detected by ELISA. The expressions of Wnt2 in breast cancer tissues and their adjacent para-cancerous tissues were observed by immunohistochemistry. The primary cells from the breast cancer cells with high- and low-expression of Wnt2 were cultured. The capacities of invasion and adhesion of the breast cancer cells with high- and low-expression of Wnt2 were determined by Transwell assay and cell adhesion experiment, respectively. Results: The serum Wnt2 content of patients with breast cancer was higher than that of the healthy volunteers (P < 0.05). The positive expression rate of Wnt2 in breast cancer tissues was higher than that in the adjacent paracancerous tissues (P < 0.01). The capacities of invasion and adhesion of breast cancer cells with high-expression of Wnt2 were higher than those of the cells with low-expression of Wnt2 (all P < 0.05). Conclusion: The expression level of Wnt2 is higher in breast cancer. The invasion capacity of the breast cancer cells with high-expression of Wnt2 is relatively stronger.