Feasibility study on plasmatic microRNA-27b-3p as a potential biomarker for early diagnosis of gastric cancer / 肿瘤
Tumor
;
(12): 183-189, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-848723
ABSTRACT
Objective:
To investigate the potential role of plasmatic microRNA-27b-3p (miR-27b-3p) as a biomarker in early diagnosis of gastric cancer (GC).Methods:
The expressions of miR-27b-3p in plasma from 46 GC patients and 40 age- and gender-matched healthy controls were detected by real-time fluorescent quantitative-PCR (RFQ-PCR). The expression of miR-27b-3p in peripheral blood cells from 12 GC patients and 12 healthy controls were also detected by RFQ-PCR. The association of plasmatic miR-27b-3p level with the clinicopathological features of GC was statistically analyzed. The sensitivity and specificity of miR-27b-3p for diagnosis of GC were evaluated by receiver operating characteristic (ROC) curve and area under the curve (AUC).Results:
The level of plasmatic miR-27b-3p in GC patients was significantly lower than that in the healthy controls (P 0.05). The downregulation of plasmatic miR-27b-3p level was correlated to the differentiation of GC (P 0.05). The ROC curve analysis showed that AUC value was 0.724, and the sensitivity and specificity of plasmatic miR-27b-3p used for differential diagnosis of GC were 60.0% and 76.1%, respectively.Conclusion:
Downregulation of plasmatic miR-27b-3p level may indicate the occurrence of GC. MiR-27b-3p may be a potential biomarker for early diagnosis of GC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Language:
Chinese
Journal:
Tumor
Year:
2015
Type:
Article
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