The role of Id1 in angiogenesis of colon cancer / 肿瘤

ABSTRACT

Objective: To explore the role and the possible mechanism of inhibitor of differentiation 1 (Id1) in angiogenesis of colon cancer. Methods: After transfection with Id1 over-expression vector plasmid, the expression levels of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) mRNAs and proteins in HT-29 cells were detected by real-time fluorescent quantitative PCR and Western blotting, respectively. The ability of tube formation of human umbilical vein endothelial cells (HUVECs) after culture with supernatant of HT-29 cells with Id1 overexpression for 24 h was observed by tube formation experiment. The tumorigenesis of HT-29 cells with Id1 over-expression in nude mice was observed, and the microvessel density (MVD) of transplanted tumor tissues was detected by immunohistochemistry, . Results: The expression levels of HIF-1α and VEGF mRNAs and proteins in HT-29 cells with Id1 over-expression were higher than those in the HT-29 cells transfected with empty vector (negative control) and the HT-29 cells without any transfection (blank control) (all P < 0.05). The number of tube formation of HUVECs after culture with supernatant of HT-29 cells with Id1 over-expression was higher than those of the negative control and the blank control groups (both P < 0.05). The ability of tmorigenesis and the number of MVD in Id1 overexpression group were both higher than those in the negative control and the blank control groups (all P < 0.05). Conclusion: Id1 may promote the angiogenesis of colon cancer by regulating the expressions of HIF-1α and VEGF.