Inhibition of proliferation of human osteosarcoma MG-63 cells by siRNA-induced down-regulation of expression of RIPK4 gene / 肿瘤

ABSTRACT

Objective: To investigate the effect of siRNA-induced down-regulation of receptor-interacting protein kinase 4 (RIPK4) gene on proliferation of human osteosarcoma MG-63 cells and expressions of Wnt/β-catenin signaling pathway-associated genes. Methods: The ostersarcoma MG-63 cells were transfected with siRNA targeting RIPK4 gene (RIPK4 siRNA), then the proliferation of MG-63 cells was examined by CCK-8 method, and the mRNA and protein expression levels of RIPK4, β-catenin and cyclin D1 were detected byreal-time fluorescent quantitative-PCR and Western blotting, respectively. Results: The proliferative ability of MG-63 cells after transfection with RIPK4 siRNA was lower than that of MG-63 cells transfected with the control siRNA (as a negative control) or without any transfection (as a blank control) (both P < 0.05). The mRNA and protein expression levels of RIPK4, β-catenin and cyclin D1 in RIPK4 siRNA transfection group were lower than those of the negative control and the blank control groups (all P < 0.05). Conclusion: Silencing the expression of RIPK4 gene can inhibit the proliferation of MG-63 cells. This effect may be related to the inhibition of Wnt/β-catenin signaling pathway.