Inhibition of expression of heparanase in hepatic cancer cell line SMMC-7721 treated with sodium cantharidinate and vitamin B6 / 肿瘤

ABSTRACT

Objective: To investigate the effects of sodium cantharidinate and vitamin B6 (SCAVB6) on cell proliferation, apoptosis and invasion of hepatic cancer cell line SMMC-7721 as well as the expression of heparanase (HPA). Methods: After treatment with 0.5, 1.0, 1.5 and 2.0 μg/mL SCAVB6 for 24, 48 and 72 h, the cell proliferation was detected by MTT assay. The cell apoptosis, invasion ability and the expression levels of HPA mRNA and protein of SMMC-7721 cells after treatment with 0.5, 1.0, 1.5 and 2.0 μg/mL SCAVB6 for 48 h were examined by flow cytometry (FCM), Transwell cell invasion assay, RT-PCR and Western blotting, respectively. Results: SCAVB6 could inhibit the proliferation of SMMC-7721 cells in a dose- and time-dependent manner (P < 0.05). The apoptotic rate of SMMC-7721 cells after treatment with SCAVB6 for 48 h was higher than that of the SMMC-7721 cells without any treatment, but the invasion ability was weakened (both P < 0.05). The expression levels of HPA mRNA and protein in SMMC-7721 cells after treatment with 1.0, 1.5 and 2.0 μg/mL SCAVB6 were lower than those in the SMMC-7721 cells without any treatment, and this inhibition effect was in a dose-dependent manner (all P < 0.05). Conclusion: SCAVB6 can inhibit the proliferation and invasion of SMMC-7721 cells. This effect may be related to the inhibition of expression of HPA.