Pituitary tumor-transforming gene inhibits the invasion ability of breast cancer cells / 肿瘤

ABSTRACT

Objective: To explore the role of pituitary tumor-transforming gene (PTTG) in invasion of breast cancer cells. Methods: The expression level of PTTG protein in selected breast cancer cells with different invasion abilities was detected by Western blotting. The expression level of PTTG protein in MDA231 cells with high invasion ability after transfection with siRNA targeting PTTG (named as siPTTG/MDA231 cells) was detected by Western blotting. The invasion ability of siPTTG/MDA231 cells was measured by Transwell cell invasion assay. The expression levels of phospho-Akt (p-Akt) and phospho-AMP-related protein kinase (AMPK)-related protein kinase 5 (p-ARK5) in siPTTG/MDA231 cells induced by epithelial growth factor (EGF) were examined by Western blotting. Results: The expression level of PTTG protein in MDA231 cells with high invasion ability was higher than those in MCF7 and T47-D cells with low invasion abilities (both P 0.05). Conclusion: The expression level of PTTG protein in breast cancer cells with high invasion ability is high. The invasion ability of MDA231 cells after inhibition of PTTG expression is decreased, and this effect may be related to the regulation of expression levels of p-Akt and p-ARK5 proteins.