Up-regulation of HSP70-2 expression mediated by dinitrosopiperazine promotes metastasis of nasopharyngeal cancer cells / 肿瘤

ABSTRACT

Objective: To investigate the mechanism of dinitrosopiperazine (DNP)-mediated heat-shock protein 70-2 (HSP70-2) expression involved in the metastasis of nasopharyngeal cancer (NPC). Methods: Non-cytotoxic concentration of DNP against NPC cell line 6-10B with low metastatic potential was determined by MTT assay. The expressions of HSP70-2 protein and mRNA in 6-10B cells treated with DNP (50 and 100 μmol/L) were detected by indirect immunofluorescence assay, Western blotting, and real-time fluorogenic quantitative-PCR, respectively. The expression of HSP70-2 was silenced by small interfering RNA (siRNA)-HSP70-2. The capabilities of invasion and migration of 6-10B cells treated with 100 μmol/L DNP were detected by Transwell assay. Results: The non-cytotoxic concentration of DNP against 6-10B cells was 0-100 μmol/L. After treatment with 100 μmol/L DNP, the expression of HSP70-2 was significantly increased and mainly located in the cytoplasm. This expression was also in a dose-dependent manner. The relative expression levels of HSP70-2 mRNA in 6-10B cells treated with 50 and 100 μmol/L DNP were 0.81 ± 0.14 and 0.81 ± 0.26, respectively, which were not significantly different from that of the blank control group (vs 1.04 ± 0.33, P > 0.05). The capabilities of invasion and migration in 6-10B cells were inhibited by silencing the expression of HSP70-2 induced by siRNA-HSP70-2. Conclusion: DNP can up-regulate the expression of HSP70-2 in 6-1 OB cells through post-transcriptional regulation mechanism and then mediate the metastasis of NPC cells. Copyright© 2014 by Tumor.