The mechanism of microRNA-29b regulating cyclin D2 expression in the genesis of human cervical cancer / 肿瘤

ABSTRACT

Objective: To analyze the expression level of microRNA-29b (miR-29b) in human cervical cancer tissues, and to investigate the function of miR-29b in regulating the genesis of human cervical cancer and its mechanism. Methods: The expressions of miR-29b in 50 tissue specimens of cervical cancer and the corresponding adjacent paracancerous tissues were detected by real-time fluorescent quantitative PCR. The relationship of miR-29b expression and clinicopathological features of patients with cervical cancer was analyzed. After the transfection of miR-29b mimics into cervival cancer SiHa cells, the effects of miR-29b overexpression on proliferation, cell cycle distribution and migration of SiHa cells were detected by cell count kit-8 (CCK-8), flow cytometry, wound-healing and Transwell assays, respectively. The target gene of miR-29b was predicted by Targetscan software. Then the interaction between miR-29b and its target gene in human cervical cancer cells was confirmed by dual-luciferase reporter gene system and Western blotting. The expression of target gene of miR-29b in cervical cancer tissues was detected by real-time fluorescent quantitative PCR. Results: Compared with the corresponding adjacent paracancerous tissues, the expression of miR-29b was down-regulated in cervical cancer tissues (P 2 = 0.225, P < 0.05) in cervical cancer SiHa cells. The expression level of CCND2 protein in cervical cancer tissues was higher than that in the corresponding adjacent paracancerous tissues (P < 0.05). Conclusion: MiR-29b is down-regulated in human cervical cancer tissues. MiR-29b may be served as a tumor-suppressor through down-regulating CCND2 expression, so it may be used as a new target for therapy and diagnosis of human cervical cancer.