The relationship between tumor stem cells marker CD90 and resistance to cisplatin in human lung adenocarcinoma A549 cells / 肿瘤
Tumor
;
(12): 770-775, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-848981
ABSTRACT
Objective:
To observe the relationship between stem cells marker CD90 and CDDP (cisplatin) resistance in lung adenocarcinoma A549 cells, and to explore whether CD90 is a marker of lung cancer stem cells.Methods:
Self-renewal abilities of A549 and A549/CDDP cells were detected by sphere-forming assay, CCK-8 (cell counting kit-8) and plate colony-formation assay, respectively. The proportions of CD90+ in A549 cells, A549/CDDP adherent cells and the spheres cells were measured by FCM (flow cytometry). The CDDP resistance in CD90+A549 cells and CD90-A549 cells was detected by cytotoxicity experiment. The CDDP resistance in CD90+A549 cells in vivo was detected by using transplanted tumor in nude mice.Results:
As compared with A549 cells, the abilities of sphere formation, proliferation and colony-formation efficiency of A549/CDDP cells were increased (P < 0.01). The proportions of CD90+ in A549/CDDP adherent cells and the sphere cells were higher than those in the corresponding A549 adherent cells and the sphere cells (P < 0.05). IC50 (half inhibitory concentration) values of CDDP in CD90+A549 and CD90-A549 cells were 8.50 and 1.50 μmol/L, respectively. The tumor xenograft volume of CD90 +A549 cells was larger than that of CD90-A549 cells after treatment with CDDP in nude mice (P < 0.05).Conclusion:
A549/CDDP cells show high self-renewal ability and CD90+ proportion. CD90+A549 cells have high ability of CDDP resistance. CD90 may be a marker of lung cancer stem cells. Copyright © 2013 by TUMOR.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tumor
Year:
2013
Type:
Article
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