The miR-200a influences the migration and invasion abilities of glioma cells by targeting NCAM1 gene / 肿瘤

ABSTRACT

Objective: To investigate the effects of miR(microRNA)-200a on migration and invasion abilities of glioma cells, and to explore its possible mechanism. Methods: Differential expression levels of miR-200a in glioma U87 cells were achieved by transfecting with hsa-miR-200a mimic, hsa-miR-200a inhibitor or hsa-miR-negative control by Lipofectamine™ 2000. The migration and invasion abilities of U87 cells were detected by wound-healing assay and Transwell invasion assay, respectively. Bioinformatics software was used to predict downstream target genes of miR-200a and their binding sites. The potential target genes were verified by Luciferase Reporter Assay and Western blotting. Results: Exogenous overexpression of miR-200a could promote migration and invasion abilities of U87 cells (P < 0.05), while miR-200a inhibitors could generate the opposite results (P < 0.05). Luciferase Reporter Assay and Western blotting revealed that hsa-miR-200a negatively regulated the protein expression of NCAM1 (neural cell adhesion molecule 1) gene which was regarded as the target gene. Conclusion: The miR-200a can promote the migration and invasion abilities of glioma U87 cells, in which NCAM1 may be one of the target genes. Copyright © 2013 by TUMOR.