Effects of gossypol acetic acid on apoptosis and DNMT1 expression of human tongue carcinoma cell line Tca8113 / 肿瘤

ABSTRACT

Objective: To investigate the effects of GAA (gossypol acetic acid) on the proliferation, apoptosis and DNMT1 (DNA methyltransferase 1) mRNA expression of human tongue carcinoma cell line Tca8113 in vitro. Methods: The proliferation and apoptosis of Tca8113 cells after treatment with different concentrations of GAA were detected by MTT and flow cytometry, respectively. The expression level of DNMT1 mRNA was examined by real-time fluorescence quantitative-PCR. Results: The proliferative abilities of Tca8113 cells were inhibited after treatment with GAA for 24, 48, and 72 h. The apoptotic rates of Tca8113 cells after treatment with 30 μmol/L GAA for 48 h and 15 μmol/L GAA for 72 h were higher than those of the control cells (without GAA treatment) (P < 0.05). The expression levels of DNMT1 mRNA in Tca8113 cells after treatment with 5, 10, 15 and 20 μmol/L GAA for 48 h were lower than those of the control cells (P < 0.05). Conclusion: GAA can inhibit the proliferation and induce the apoptosis of human tongue carcinoma cell line Tca8113 with a decreased expression level of DNMT1 mRNA. Copyright © 2013 by TUMOR.