The correlation among hMLH1, NIS and TSHR genes with promoter methylation in papillary thyroid carcinoma and its clinical significance / 肿瘤

ABSTRACT

Objective: To investigate the aberrant methylation status of hMLH1 (human mutL homologue 1) gene in PTC (papillary thyroid carcinoma) tissues, and its correlations with the aberrant methylation of NIS (sodium iodide symporter) and TSHR (thyroid-stimulating hormone receptor) genes. Methods: qMSP (quantitative methylation-specific PCR) was carried out to detect the promoter methylation status of hMLH1, NIS and TSHR genes in PTC tissues and adjacent normal thyroid tissues from 152 patinets with PTC. The correlation between the clinicopathologic characteristics and the promoter methylation status of hMLH1 gene was analyzed. The relationships among the methylation status of hMLH1, NIS and TSHR genes and their independent or synergistic effects on the progression of PTC were investigated. Results: The promoter methylation rate of hMLH1 gene in PTC tissues (37.5%) was significantly higher than that in the adjacent normal thyroid tissues (5.3%) (P 0.05). There was a weak correlation among aberran promoter methylation of hMLH1, NIS and TSHR genes (r 0.05). Conclusion: The aberrant methylation of hMLH1 gene promoter in PTC tissues might be associated with the progression of the tumor. There exists no correlation among the aberrant methylation of hMLH1, NIS and TSHR genes, and their independent or synergistic effect also may not be associated with the progression of PTC. Copyright © 2013 by TUMOR.