The effects of 5-aza-2'-deoxycytidine on proliferation and cell cycle of MCF-7/ADR cells and KBV/200 cells and their mechanism / 肿瘤
Tumor
;
(12): 874-879, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-849029
ABSTRACT
Objective:
To investigate the effect of 5-Aza-dC (5-aza-2'-deoxycytidine) on cell cycle of multidrug resistant cells and its mechanism.Methods:
Inhibitory effects of 5-Aza-dC on proliferation of breast cancer MCF-7/ADR cells and oral epidermoid carcinoma KBV/200 cells were detected by MTT method. The changes of cell cycle distribution were examined by flow cytometry when the cells were treated with 5-Aza-dC at different concentrations for 72 h or treated with the same concentration of 5-Aza-dC (10 μmol/L) for different time. The expression levels of cyclin A, cyclin E and p21waf1/cip1 mRNAs and proteins were detected by real-time fluorogenic quantitative-PCR and Western blotting, respectively.Results:
The proliferation of MCF-7/ADR and KBV/200 cells was suppressed significantly after 5-AzadC treatment. The cell cycle was arrested at G2/M phase in dose- and time-dependent manners. The expression levels of cyclin A, cyclin E and p21waf1/cip1 mRNAs and proteins were elevated.Conclusion:
The cell cycle of MCF-7/ADR and KBV/200 cells can be arrested at G2/M phase which consequently results in the inhibition of proliferation of multidrug resistant cells induced by 5-Aza-dC. The molecular mechanisms may be related with up-regulation of expressions of cyclin A, cyclin E and p21waf1/cip1 mRNAs and proteins. Copyright © 2012 by TUMOR.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tumor
Year:
2012
Type:
Article
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