Small interference RNA-mediated CYP 1B 1 gene silencing inhibits the proliferation of human breast cancer MDA-MB-231 cells induced by polyunsaturated fatty acids / 肿瘤
Tumor
;
(12): 429-434, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-849078
ABSTRACT
Objective:
To investigate the effect of CYP 1B 1 (cytochrome P-450 1B1) gene silencing induced by small interference RNA (siRNA) on the proliferation of MDA-MB-231 cells treated with EPA (eicosapentaenoic acid) and AA (arachidonic acid).Methods:
The proliferation of MDA-MB-231 cells treated with EPA or AA was detected by CCK-8 (cell counting kit-8) assay. The expression of CYP1B1 was interfered by RNAi (RNA interference) technique. The transfection efficiency was examined by realtime fluorescence quantitative PCR (RFQ-PCR) and Western blotting, respectively. The expression levels of CYP1B1 and COMT (catechol-O -methyltransferase) mRNAs and proteins in MDA-MB-231 cells interfered with siRNA and treated with EPA or AA were determined by RFQ-PCR and Western blotting, respectively. The viability of breast cancer MDA-MB-231 cells interfered with siRNA and treated with EPA or AA was detected by CCK-8 assay.Results:
The cell number of EPA-treated group was lower while the cell number of AA-treated group was higher than that of the control group (P <0.05). The expression levels of CYP1B1 mRNA and protein were decreased in MDA-MB-231 cells transfected with CYP1B1 siRNA, while the expression levels of COMT mRNA and protein were increased. The proliferation of MDA-MB -231 cells transfected with CYP1B1 siRNA was inhibited, and the number of MDA-MB -231 cells treated with EPA was significantly higher than that of the negative control group (P <0.05).Conclusion:
CYP 1B 1 gene silencing inhibits the proliferation of MDA-MB-231 cells and reverses the inhibitory effect of EPA on the cell proliferation. EPA probably inhibits the cell proliferation through regulating the expression of CYP1B1 in breast cancer. © 2012 by Tumor.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tumor
Year:
2012
Type:
Article
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