iTRAQ analysis of serum biomarkers of hepatocellular carcinoma based on mass spectrometry / 肿瘤

ABSTRACT

Objective: The technique of isobaric tags for relative and absolute quantitation (iTRAQ) was used to analyze the expression levels of serum biomarkers of hepatocellular carcinoma (HCC) based on mass spectrometry. Methods: The literature about HCC biomarkers identified by screening of differential protein expression combined with matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS) published between January 2003 and August 2010 were selected by searching PubMed database. Screening for serum differential proteins was performed in 20 HCC patients and 20 healthy volunteers by using iTRAQ labeling and MALDI-TOF-MS. The reported data of HCC biomarkers were compared with the screening results of serum differential protein expression in HCC. Results: Twenty-two pieces of eligible literature were obtained by information retrieval, involving 262 HCC biomarkers. Fifty-one proteins were identified having differential expression by using screening of serum differential protein expression in HCC. In the 51 proteins, the expression level of 23 proteins was high, while the expression level of 28 proteins was low. Eight differential proteins which were identified by screening, were in accordance with literature reports, and 6 differential proteins (alpha-1-antitrypsin, apolipoprotein E, ceruloplasmin, alpha fetoprotein, complement C3 and serum amyloid P-component) had the similar expression levels in accordance with literature reports. Conclusion: Six differential proteins identified in serum are the same as reported in the literatures, and these proteins may become potential HCC biomarkers. There is a need for further study to explore these differential proteins. Copyright© 2011 by Tumor.