Loss of heterozygosity and abnormal expression of MTSS 1 in the progression and metastasis of advanced primary gastric carcinoma / 肿瘤

ABSTRACT

Objective: To determine the correlation of metastasis suppressor 1 (MTSS 1) gene with the prognosis of gastric cancer by detecting the expression level of MTSS 1 in gastric cancer, and to explore its underlying molecular mechanism in the development and progression of gastric cancers. Methods: The expression levels of MTSS1 in the primary lesion, adjacent normal mucosa tissue, lymph node metastasis and other metastatic lesions from patients with gastric cancer were detected by using immunohistochemistry and RT-PCR, and the correlation of MTSS 1 expression with the clinicopathological factors was evaluated. The loss of heterozygosity (LOH) of four microsatellite loci (D8S1832, D8S2132, D8S1179 and D8S1461) in MTSS 1 gene in thirteen patients with negative-expression of MTSS1 was detected by using PCR-polyacrylamide gel electrophoresis-silver staining method. Results: The positive expression rates of MTSS 1 were 33.9% (42/124) in the primary lesions and 16.3% (8/49) in the lymph node metastasis and other metastatic lesions. Loss expression of MTSS 1 was significantly associated with the advanced T stage, lymph node metastasis, advanced disease stage, and the poor histological differentiation. Loss or down-regulation of MTSS 1 expression was significantly correlated with the poor survival rate in both univariate and multivariate analysis. A higher frequency of allelic loss was observed in the primary (46.2%) or metastatic (69.2%) gastic cancer lesion with negative expression of MTSS 1. Conclusion: The loss and decreased expression of MTSS 1 may play an important role in the development and progression of gastric cancers, and MTSS 1 gene may become a potential predictor for clinical outcome of patients with gastric cancer. Copyright© 2011 by the Editorial Board of Tumor.