Inhibitory effects of curcumin on human multiple myeloma cell lines and their mechanisms / 肿瘤
Tumor
;
(12): 1051-1054, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-849240
ABSTRACT
Objective:
This study was designed to explore the effects of curcumin on the proliferation and apoptosis of human multiple myeloma cell lines H929 and RPMI8226, and to investigate their relevant mechanisms.Methods:
MTT assay was used to detect the inhibitory effect of curcumin on cell proliferation. Flow cytometry (FCM) was applied to study cell cycle distribution and apoptosis rate changes. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of survivin, Bcl-2, and Bax.Results:
Curcumin inhibited the proliferation of myeloma cells H929 and RPMI8226 in a time- and concentration-dependent manner. Curcumin induced apoptosis of RPMI8226 and arrested RPMI8226 cells at G2/M phase. Curcumin significantly down-regulated mRNA expression of survivin and Bcl-2 in both H929 and RPMI8226 cells, while markedly up-regulated Bax mRNA expression.Conclusion:
Curcumin inhibites the proliferation and induces the apoptosis of human multiple myeloma cells. This work supposes that the effect of curcumin on transcriptions of survivin, Bcl-2 and Bax might be one of the mechanisms underlying tumor inhibitory effects of curcumin.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tumor
Year:
2008
Type:
Article
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