Inhibitory effects of curcumin on human multiple myeloma cell lines and their mechanisms / 肿瘤

ABSTRACT

Objective: This study was designed to explore the effects of curcumin on the proliferation and apoptosis of human multiple myeloma cell lines H929 and RPMI8226, and to investigate their relevant mechanisms. Methods: MTT assay was used to detect the inhibitory effect of curcumin on cell proliferation. Flow cytometry (FCM) was applied to study cell cycle distribution and apoptosis rate changes. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of survivin, Bcl-2, and Bax. Results: Curcumin inhibited the proliferation of myeloma cells H929 and RPMI8226 in a time- and concentration-dependent manner. Curcumin induced apoptosis of RPMI8226 and arrested RPMI8226 cells at G2/M phase. Curcumin significantly down-regulated mRNA expression of survivin and Bcl-2 in both H929 and RPMI8226 cells, while markedly up-regulated Bax mRNA expression. Conclusion: Curcumin inhibites the proliferation and induces the apoptosis of human multiple myeloma cells. This work supposes that the effect of curcumin on transcriptions of survivin, Bcl-2 and Bax might be one of the mechanisms underlying tumor inhibitory effects of curcumin.